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Winter Hardiness and Changes in Soluble Protein Fractions of Bermudagrass¹

Regrowth data taken following a freezing test indicated that two bermudagrass (Cynodon sp.) cultivars gradually increased in winter hardiness during the autumn and early winter months. Maximum resistance, which represented a change of approximately 6.0 C, was reached during January and February. A decrease in hardiness was observed as the grass initiated new growth in the early spring.

Polyacrylanfide gel electrophoresis was used to characterize the changes in soluble protein fractions in the overwintering parts. When plants were exposed to a hardening environment for more than 15 days, two of the bands present in nonhardened material decreased in density and were more widely separated. Other protein changes during overwintering included the appearance of four cempact and well defined bands near the origin. These changes may reflect alterations in the metabolic systems that are related to the hardening process. Comparable data were obtained in field and growth chamber studies.

Key Words: Turfgrass • Cold resistance • Freezing test • Polyacrylamide gel electrophoresis

² Assistant Professor of Agronomy, University of Kentncky, Lexington, Ky., 40506, and Associate Professor of Crop Science, North Carolina State University, Raleigh, N. C. 27607.

Received for publication September 12, 1968.

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