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Five cultivars and two experimental lines were used to evaluate stomate density and length in alfalfa (Medicago sativa L.). Plants were grown under similar conditions with respect to light, temperature, and soil-moisture conditions. Stomate impressions were taken on the adaxial and abaxial leaf surfaces at four leaf positions on plants from all cultivars and experimental lines used in this study.
Average stomate density ranged from 146 per mm2 for Sonora to 265 per mm2 for Ladak. Stomate density was significantly greater on the adaxial leaf surface compared to the abaxial surface. Terminal leaves of all cultivars and experimental lines had more stomata per unit area than basal leaves.
Key Words: Leaf position and stomate density
2 Research assistant and Associate Agronomist, Department of Agronomy, University of Arizona, Tucson. 85721.
Received for publication June 15, 1969.
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