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Three major glucosinolates "gluconapin," "glucobrassicanapin," and "progoitrin" were quantitatively determined in rapeseed meal for three pod positions on plants of the varieties Nugget (Brassica napus L.) and Echo (B. campestris L.) grown in three environments. Significant position effects were found for all three glucosinolates in Nugget, but only for gluconapin in Echo when position effects were averaged for three environments. However, within environments the pod position effects did not always follow these patterns. Single pod samples should be restricted to the same position on each plant to reduce the random variation in glucosinolate content. The effect of environment on the glucosinolate content of seed from plants grown under three environments was significant. Comparisons of glucosinolate values for plants grown at different times or locations should be avoided.
Key Words: Environmental effects Isothiocyanates Oxazolidinethione
2 Plant Science Department, University of Alberta, Edmonton, and Canada Department of Agriculture Research Station, Saskatoon, Saskatchewan, respectively.
Received for publication May 23, 1969.
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