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Published in Crop Sci 10:13-14 (1970)
© 1970 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Serological Evaluation of Winter Wheat Aneuploids1

R.B. Volin and J.R. Welsh2

Antigenic components from the leaves of several monosomic (2n - 1) and disomic (2n) plants from each of 18 monosomic lines of winter wheat, Triticum aestivum L. em. Thell. (aestivum group) ‘Kharkof MC22’ (2n = 42), were compared serologically.

Antibodies were produced in rabbits by injecting them with leaf extract antigens from two sources: (1) a disomic which was derived from segregating monosomic and disomic lines of the variety Kharkof MC22 and (2) a single plant selection from Kharkof MC22. Two lots of crude sap and two lots treated with ammonium sulfate saturation methods were used.

Gel-diffusion techniques revealed no differences between the different antigens or between the monosomic and disomic antigens when reacted against any one antiserum. Precipitation lines of partial or non-identity were not present. No antibodies remained following antiserum absorption with monosomic plant extract.

No differences could be found between antigens inherent in the monosomic and disomic genetic conditions when compared serologically.

Key Words: Monosomic • Antigen • Antiserum

1 Contribution of the Plant and Soil Science Department, Montana State University, Bozeman 59715. Published with the approval of the Director of the Montana Agr. Exp. Sta. as Paper No. 970. Journal Series

2 Graduate Research Assistant, Botany and Microbiology Department (Formerly Graduate Research Assistant, Plant & Soil Science Department), Montana State University, and Associate Professor, Agronomy Department, Colorado State University, Ft. Collins, 80521 (formerly Associate Professor, Plant & Soil Science Department, MSU).

Received for publication January 24, 1968.




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