Figure 5. Relative expression levels of four validated Brassica orthologs of Arabidopsis genes in functional categories found to be strongly responsive to priming treatment, assayed by real-time quantitative reverse transcription polymerase chain reaction (RT–PCR). The data represent the mean of five replicates, with error bars representing the standard error of the mean. The five points on each curve correspond to (1) untreated seed, (2) primed seed, (3) primed and dried seed, (4) primed seed after shelf-life induction, and (5) primed seed after shelf-life induction followed by drying. The expression levels were normalized first to actin measured from the same RNA sample and then to the mean of all points for each genotype.