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Figure 3


Figure 3. Optimization of direct hybridization (DH) assay. SNP358 marker was genotyped across the F2:3 population from the cross of PI 96354 x Bossier using different length of probes and different hybridization temperature conditions. Data are presented as the log10 [(fraction of PI 96354 allele signal + 0.01)/(fraction of Bossier allele signal + 0.01)] versus total mean fluorescence intensity (MFI) of two alleles (Hirschhorn et al., 2000). Genotypes are represented by homozygotes for PI 96354 allele (Figure 3), heterozygotes (Figure 3), and homozygotes for Bossier allele (Figure 3) determined by the band sizes of the Satt358 SSR marker. (H.T. = hybridization temperature; ASO = allele-specific oligonucleotide).