Figure 6. Glycine max F3'5'H gene polymorphisms and copy number. A DNA gel blot showing the hybridization of a genomic F3'5'H probe (3.5 kb) to DNAs isolated from three pairs of W1 isolines digested with HindIII and/or PstI. The smaller size HindIII fragment shows the 53-bp size difference between the W1 and w1 lines both in the single (HindIII) and double (HindIII and PstI) digested samples. HindIII cuts the F3'5'H gene in exon 3; the hybridization pattern shows the resulting two fragments in samples digested with this enzyme. PstI does not cut the F3'5'H gene.