Figure 6. Northern blot analysis of CBF3 transcripts after T2 plants derived from Line 28 were treated with different low temperatures. Plants were grown at 26°C and then were gradually exposed to low temperatures (20, 16, 12, 10, 4, 0, and –2°C), 3 h for each temperature. Full length of the coding region of CBF3 was used as a probe and actin was used as a loading control.