Figure 4. Southern blot analysis of T₀, T₁, and T₂ CBF3 plants: (A) Southern blot analysis of T₀ plants, showing Lane 1—kb ladder; Lane 2—untransformed plant digested with KpnI; Lanes 3, 5, and 7—Lines 7, 8, and 28 digested with Hind III; Lanes 4, 6, and 8—Lines 7, 8, and 28 digested with KpnI; and Lane 10—plasmid positive control digested with KpnI; (B) T₁ plants that originated from Line 28, showing Lane 1—kb ladder, Lane 2—untransformed plant; Lanes 3 to 9—plants no. 1, 3, 4, 7, 11, 13, and 14 digested with KpnI; and Lane 11—plasmid positive control digested with KpnI; (C) T₂ plants digested with KpnI, showing Lane 1—kb ladder; Lane 2—untransformed plant; Lanes 3 to 20—T₂ plants no. 1 to 18; and Lane 21—plasmid positive control.