Figure 2. Relationships between zoysiagrass freeze tolerance (LT₅₀, the lethal temperature killing 50% of the plants) and (A) the change in total soluble sugars during acclimation (sugars) (r² = 0.31, P = 0.05), (B) starch concentration in cold-acclimated plants (r² = 0.37, P = 0.03), (C) the sugar/starch ratio in cold-acclimated plants (r² = 0.45, P = 0.01), and (D) the change in the sugar/starch ratio (sugar/starch) during acclimation (r² = 0.44, P = 0.01). Each point represents the mean of six replications. *Significant at the 0.05 probability level.