Figure 5. (A) Immunoblot of a 25-kDa dehydrin-like polypeptide from zoysiagrass genotypes with different freeze tolerances, showing a 25-kDa dehydrin probed with a 1:250 dilution of antidehydrin polyclonal primary antibody for cold-acclimated plants. Equal amounts of protein (25 µg) were loaded in each lane. (B) There was no correlation (r = 0.47, P = 0.11) between freeze tolerance (LT₅₀, the lethal temperature killing 50% of the plants) and optical density (O.D.) of the 25-kDa dehydrin band.