Figure 2. Molecular mapping of rust-resistant introgression T2BS·2BL-2^tL(0.95) using bulked segregant analysis. Southern hybridization pattern of EcoRV-digested genomic DNA of parents and bulks from homozygous resistant and susceptible F₂ plants using probe KSUH16. The restriction fragment length polymorphism fragments diagnostically polymorphic between resistant and susceptible bulks are indicated by arrows. The WL711 allele of XksuH16 replaced by an Aegilops triuncialis-specific allele is indicated with an asterisk.