Figure 2. An allele-specific assay to distinguish between PI 361088B and wild-type GmFAD3A alleles. (A) Sequence of nucleotides 301 to 330 of the GmFAD3A coding sequence from ‘Williams 82’ and PI 361088B DNA. Amino acid sequence is displayed below their corresponding codons. The position of the forward primers used in the assay is indicated by a black bar above the corresponding sequence. A dashed line is used where sequence is not shown. The "long GC" and "short GC" 5' tails are also indicated. (B) Melting curve analysis on Williams 82 and PI 361088B PCR products from the allele-specific polymerase chain reaction. The melting curve shows a melting temperature of 79.8°C for PI 361088B and 82.2°C for Williams 82.