Figure 3. Southern blot analysis of transgenic bahiagrass. Genomic DNA (20 µg) from nontransgenic wild-type (WT) and transgenic bahiagrass lines (4–52) was digested with BglII, which cuts once in the expression cassette of the bar gene (Fig. 1). The full length coding region of the bar gene was used as a probe and labeled with ³²P-deoxy cytidine 5'-triphosphate. Linearized pJFBar plasmid (20 pg) was used as a positive control (PC).