Figure 1. BLAST (NCBI, 2006) and ClustalX (Thompson et al., 1997) sequence alignment of nucleotide sequences from (A) TmPina5A (T. monococcum Pina-A^m1) and TaPina5D (T. aestivum Pina-1), (B) TmPinb5A (T. monococcum Pinb-A^m1) and TaPinb5D (T. aestivum Pinb-1), and (C) TmGsp5A (T. monococcum Gsp-A^m1) and TaGsp5A, 5B, 5D (T. aestivum Gsp-1). Primer sequences for sequence tagged site markers Pina-A^m1, Pinb-A^m1, and Gsp-A^m1 are in underlined bold type. Shaded letters in DNA sequences correspond to the point mutations/insertion between T. monococcum and T. aestivum. Gel lanes: 1, DNA ladder; 2, donor of soft-textured grain (CS-5A^m); 3, Chinese Spring; 4–26, BCF₁ segregating population; 27, DNA ladder. Arrows indicate the T. monococcum–specific bands.