Fig. 4. Western Blot analysis of R5 transgenic and nontransgenic soybean lines. Total protein was extracted from leaves and digested with trypsin. Denatured protein was separated by sodium dodecyl sulfate–polyacrylamide gel electroporesis, blotted, and visualized using Cry1Ac antiserum and chemiluminescent detection system. The soybean line A3237 was the recurrent parent in all material examined: (1) 862, (2) 726, (3) 781, (4) A3237 soybean genotype (negative control), (5) negative segregent for line 862.