Fig. 3. Comparison of Wx gene expression levels among the selected lines and hybrids, and their originals by both transcript (A and B) and protein levels (C). Northern blot analysis of hybridized signals with the Wx probe. The 3.4 kb Wx pre-mRNAs and 2.3 kb Wx mature mRNAs are indicated by arrows (panel A). Panel B shows the rRNAs by EB staining after electrophoresis; SDS-PAGE of Coomassei blue stained Wx proteins purified from the endosperm of mature seeds (panel C). The genotypes of PCR-AccI molecular marker of the analyzed samples is shown in panel D. [Lane 1: LTF-B original; lanes 2–6: five individual plants in BC₄F₂ generation; lanes 5–6 represent the two selected lines L-tt-1 B and L-tt-3 B; lane 7: 9311; lane 8: ZS-B original; lane 9–10: two selected lines Z-tt-1 B and Z-tt-2 B; lane 11: YH559; lane 12: original hybrid Teyou 63; and lane 13: one new hybrid LTF (tt)-A-1xMH63.]