Fig. 2. Determination of the PCR-AccI molecular marker in individuals of the BC₄F₂ generation from two target parents, LTF-B (A) and ZS-B (B). Panel (A) lane 1: 9311 (TT genotype); lane 2: LTF-B (GG genotype); lanes 3–12: individuals in BC₄F₂ generation; and lane 13: hybrid Teyou 559 (GT genotype). Panel (B) lane 1: YH559 (TT genotype); lane 2: ZS-B (GG genotype); lanes 3–12: individuals in BC₄F₂ generation; and lane 13: Shanyou 63 (GT genotype). Lane M: the 1 kb plus DNA molecular marker (Invitrogen).