Fig. 5. Sequential SDS-PAGE separation of gliadins (A) and high molecular weight glutenins (B) from the same kernels of various triticale lines. Lanes 1: Presto, 2: Presto Dt1RL, 3: Presto 1R.1D₅₊₁₀-2, 4: Presto 1D(1R), 5: Presto Valdy, 6: 1RS_64:4.1DL in an early backcross to Presto; Glu-A1 and Gli-B1 alleles of Pavon are present, 7: Presto FC1, 8: Presto RM1. Chromosome 1D in substitution for 1R (lane 4) carries Glu-D1)(a) (subunits 2+12). There is a clear difference in the patterns of bands in Gli-B1 of Presto (lanes 1, 2, 3, 4, 5, and 7) and that of Pavon (lanes 6 and 8–lane 8 has both alleles present, that of Presto present on chromosome 1B and that of Pavon present on chromosome RM). Photo courtesy of Dr. W. Brzezinski.