Fig. 7. Western blot of heat-stable proteins immunologically reacting with dehydrin antibody for a) developing and b) mature soybean seeds from plants exposed to irrigated (I) and nonirrigated (NI) (rainfed) conditions at beginning seed fill (R₅) in the field experiment. Equal weight of protein (7 µg) was loaded per lane. The developing seeds were sampled at 0 (R₅), 5 (R_5.4), 15 (R_5.7), 22 (R₆), 43 d (physiological maturity [PM]) after the stress treatments were imposed at beginning seed fill (R₅). Mature seeds were sampled at harvest maturity when seeds dried in the field. The lane on the left side indicates the molecular weights (MW) of standard proteins (SP). The lane on the right is used to specify MW of proteins indicated by arrows.