Fig. 6. SDS PAGE of heat-stable proteins for a) developing and b) mature soybean seeds from plants exposed to irrigated (I) and nonirrigated (NI) (rainfed) conditions at beginning seed fill (R₅) in the field experiment. Equal weight of protein (7 µg) was loaded per lane. The developing seeds were sampled at 0 (R₅), 5 (R_5.4), 15 (R_5.7), 22 (R₆), 43 d (physiological maturity [PM]) after the stress treatments were imposed at beginning seed fill (R₅). Mature seeds were sampled at harvest maturity when seeds dried in the field. The lane on the left side indicates the molecular weights (MW) of standard proteins (SP).