Fig. 5. Southern blot analysis of some of the primary transgenic plant lines. (A) Transgenic plant lines with Allium cepa agglutinin (ACA) gene. Lane 1, untransformed plant as negative control (–ve); lanes 2–8, transgenic lines ACA#7, 6, 8, 16, 2, 10, 12, respectively; lane 9, plasmid DNA (pCAMBIA-ACA) as positive control (+ve). (B) Transgenic plant lines with ASAL::ACA fused lectin gene; lane 1, untransformed plant as negative control (–ve); lanes 2–9 transgenic lines ASAL::ACA# 2, 4, 5, 7, 6, 8, 9, 11, respectively; and lane 10, plasmid DNA (pCAMBIA- ASAL::ACA) as positive control (+ve). ASAL, Allium sativum leaf agglutinin.