Fig. 4. Western blot analysis of total protein from transformed B. juncea plants using Allium cepa agglutinin (ACA) specific antibody. (A) Expression of ACA gene product amongst transgenic lines (T0). Lane 1, untransformed plant (–ve); lane 2, ACA peptide as positive control (+ve); lanes 3–9, transgenic plant lines ACA#2, 16, 12, 8, 14, 18, and 4, respectively. (B) Expression of fusion lectin amongst transgenic lines (T0) ASAL::ACA. Lanes 1–7, transformed plants with ASAL::ACA#2, 4, 5, 8, 9, 7, 11, respectively; lane 8, untransformed plant as negative control (–ve); lane 9, ACA peptide as positive control (+ve). ASAL, Allium sativum leaf agglutinin.