Fig. 4. Association of linolenic acid phenotype with genotype in plants segregating for mutant alleles of GmFAD3B and GmFAD3C derived from a backcross (SS97–6946 x (SS97–6946 x IA3017)). Fifty BC₁F_2:3 seeds produced on a aaBbCc plant (26 g kg^–1 of oil) were chipped for fatty acid analysis with the remainder of the seed germinated and then subjected to genotyping for A29 or wild-type alleles of GmFAD3B and GmFAD3C. The samples were pooled by genotype, as listed. The number of lines represented in each pool follows the genotype in parentheses. No lines were recovered with the complete mutant genotype. Histograms represent mean linolenic acid (g kg^–1 of oil) plus one standard deviation from the mean.