Fig. 2. Identification of a mutation in the A29 allele of GmFAD3C. (A) Amino acid alignment of a portion of the soybean and Arabidopsis FAD3 protein sequences (At2 g29980, GenBank accessions AY204710, AY204711, and AY204712). Identical amino acids are highlighted in black while similar amino acids are highlighted in gray. The wild-type and A29 GmFAD3C alleles are shown from amino acid 280 to 329. A SNP in the coding sequence results in a H304Y mutation (indicated above the alignment with an asterisk) for A29 GmFAD3C. Histidine-rich region II (Shanklin et al., 1994) is underlined. (B) Sequence of genomic DNA from Williams 82 GmFAD3C beginning at base 864 and ending at base 951 of the cDNA sequence. Only exon sequences are in the listed region. The base mutated in A29 is underlined (base 910 of the coding sequence) and the BccI recognition sequence is in parentheses.