Fig. 3. Extraction of (A) TX114 soluble and (B) friabilin or starch-bound puroindoline proteins from six transgenic lines derived from crosses of Heron (Pina-D1a/Pinb-D1a) with Hi-Line (Pina-D1b/Pinb-D1b) transgenic isolines with added Pina (HGA), Pinb (HGB) or both Pina and Pinb (HGAB) and hard and soft wheat controls. The PINA and PINB proteins are marked with arrows. All HG line extracts were prepared from F₄ seeds homozygous for the Pin transgene and the native Pinb-D1a allele inherited from Heron. The Heron ladder serves as a loading control with all other samples loaded at 1x for comparison to Heron.