Fig. 2. Frequency distribution for grain hardness of progeny derived from crosses of Heron (Pina-D1a/Pinb-D1a) with Hi-Line (Pina-D1a/Pinb-D1b) transgenic isolines with added Pina (HGA), Pinb (HGB), or both Pina and Pinb (HGAB), where progeny were determined homozygous for the presence of the added transgene and the Pin-D1a allele inherited from Heron. From top to bottom, the three histograms compare the following: (A) HGA1 vs. HGA3, which express additional Pina, (B) HGB5 vs. HGB12, which express additional Pinb, and (C) HGAB18 vs. HGAB3, which express additional Pina and Pinb. SKCS values were the mean of F2 plant and replicated 3-m rows grown in rainfed and irrigated environments.