Fig. 1. Cleaved amplified polymorphism sequence (CAPS) test used to distinguish between Pinb-D1a (Heron allele) and Pinb-D1b (Hi-Line allele) at Ha. BsrBI digests the Pinb-D1a PCR product once (340- and 129-bp products) and the Pinb-D1b PCR product twice (245-, 129-, and 95-bp products). The "undigested" lane was loaded with undigested Pinb-D1a PCR product. MW represents a 1-kb molecular weight standard (Promega, Madison, WI).