Fig. 1. Panel A; Lanes 1– 6. Fragments generated when the microsatellite gwm538 associated with Karnal bunt resistance was used to evaluate genotypes HD29 (resistant parent), WL711 (susceptible parent) and KB RILs #31 and #134 (heterozygotes). Panel B; Lanes 7– 12. Fragment profiles for HD29(R), WL711(S) and KB RILs #31 and #134 (H) generated with the SNP marker. Panel C; Lanes 13– 18. Physical mapping of microsatellite gwm538 fragments in Chinese Spring (control), N4AT4D (nullisomic for 4A), N4BT4D (nullisomic for 4B) and N4DT4B (nullisomic for 4D). Size standard HyperLadder IV (Bioline, Randolph, MA), with 100- and 200-bp markers indicated in Lanes 1, 7, and 13. Negative (no template DNA) controls in Lanes 6, 12, and 18.