Fig. 5. Limits of N. coenophialum detection in tall fescue seed. DNA was isolated from tall fescue cv. Kentucky 31, E+ seed (90%) and E– seed (0%). The percentages above the lanes represent the final proportions of DNA isolated from E+ (90%) seed combined with the DNA isolated from E– seed (0%). Approximately 1 ng of isolated DNA/reaction. MW, Invitrogen 1 kb plus DNA ladder.