Fig. 3. Determination of DNA concentration necessary for PCR detection of Neotyphodium sp. Numbers above the lanes represent the amount of DNA used in the PCR reaction. MW- Invitrogen 1 kb plus DNA ladder. (A) DNA isolated from N. coenophialum fungus isolated from tall fescue. (B) DNA extracted from 20 E+ seeds (tall fescue cv. Kentucky 31, 90% infected).