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Fig. 2. Principal coordinate analysis based on modified Roger's distance. PC1, PC2, and PC3 are the first, second, and third principal coordinate, respectively. CIMMYT maize lines extracted from three pairs of heterotic populations used in the subtropical hybrid program are Pop33 ({square}) x Pop45 ({blacksquare}), Pop42({circ}) x Pop44 (•), and Pop501 ({Delta}) x Pop502 ({blacktriangleup}).