Fig. 3. Cleaved amplified polymorphic sequence marker for the MIPS1S gene. A 458-bp MIPS1S amplification product, obtained using the Zm1302 + Zm1580 primers, showed MscI restriction differences that were able to distinguish –/– lpa241 MIPS1S ACR gene (fragments, 292 bp + 166 bp) from +/+ Lpa241 MIPS1S B73 allele (fragments, 133 bp + 159 bp + 166 bp).