Fig. 2. Southern blot analysis for insert and copy number. Ten micrograms of MON846 DNA (A1xA634) (lane 1) and MON863 DNA (Lanes 2 and 5) were digested with NdeI (Panel A) or EcoRV (Panel B). Plasmid PV-ZMIR13 DNA (Lanes 3 and 4) was spiked into 10 µg of MON846 DNA and digested with NdeI and EcoRV (Panel A) or EcoRV (Panel B). High molecular weight DNA ladder (Gibco BRL, Gaithersburg, MD) was loaded on the long run and molecular weight markers II and IX (Roche, Indianapolis, IN) were loaded on the short run in both panels. The arrows denote the sizes obtained from the molecular weight markers on the ethidium-stained gel.