Fig. 2. Puroindoline proteins, PINA and PINB, were fractionated via sodium dodecyl sulphate polyacrylamide gel electrophoresis and can be resolved into two bands around 15 kDa. The negative durum control ‘Langdon’ has neither PINA nor PINB, whereas both are present in the positive control line Langdon-5D(5B) denoted as L5D.