Fig. 3. Undigested and PstI digested PCR amplification products with CAPS primers S30-13L and AGA7-759R. 1-3) Chinese Spring nullisomic-tetrasomic lines. 1) N1AT1B, 2) N1BT1D, 3) N1DT1A, 4) Neepawa, 5) Neepawa*6/Triticum speltoides F-7-3. The gray arrowhead indicates the 780-bp product band from the B genome. The black arrowhead indicates the PstI digested product from the S genome. "M" indicates the molecular markers in bp (100-bp ladder, Biodynamics Corp., Seattle, WA).