Fig. 2. Best-fit alignment of partial nucleotide sequences from wheat clones from the A (pNF7-17), D (pNB-25), S (pNF7-16), and B (pNee-2) genomes. Gaps were introduced to maximize nucleotide alignment and are indicated with dashes. Locations of PCR primers AGA7-342F and S30-13L are indicated with arrows and sequences from the primers are italicized. BamHI and PstI restriction sites used to develop the CAPS marker are underlined with letters in gray color. Exons are numbered from the first transcribed exon and indicated above the sequences.