Fig. 2. Negative image of the simple sequence repeat marker Stm0020 fragments separated in 3% (w/v) Metaphor agarose gel run at 100 V for 4.5 h. A) S. microdontum (resistant parent), MSA133-57 (susceptible parent), bulks and ten resistant and susceptible progeny clones, respectively. Stm0020b linked with resistance and Stm0020a linked with susceptibility, both from S. microdontum. B and C) SSR marker Stm0020b followed through polyploidization. Parents of the diploid population, diploid resistant clone of the progeny (DLB1-150) and tetraploid parents with respective tetraploid progeny clones.