Fig. 7. Transgene stability at the NK603 integration site. Corn genomic DNA (10 µg) was digested with EcoRV, electrophoresed in a 0.8% (w/v) agarose gel, transferred to a nylon membrane, hybridized to a 32P-labeled probe from the CTP/CP4 EPSPS portion of PV-ZMGT32, and subjected to autoradiography. Individual lanes: 1, B73 nontransgenic control; 2, LH82 x NK603 B73 BC1; 3, NK603 B73 BC1F1; 4, LH82 x NK603 B73 BC2F3; 5, LH59 x NK603 B73 BC4F4. Molecular weight markers are indicated on the side of the autoradiogram (kb, kilobase pairs).