Fig. 5. Western blot analysis of CP4 EPSPS protein expressed over five generations of NK603. Total protein was extracted from NK603 leaves. Denatured protein was separated by SDS-PAGE, blotted, and visualized by means of a chemiluminescent detection system and CP4 EPSPS antiserum. The corn inbred B73 was used as a recurrent parent in all generational material examined except for hybrid samples which utilized a cross to the LH82 inbred. Lane: 1) molecular weight markers (dye-linked markers visible on blot); 2) 5 ng of E. coli-expressed CP4 EPSPS; 3) 2.5 ng of E. coli-expressed CP4 EPSPS; 4) 1 ng of E. coli-expressed CP4 EPSPS; 5) buffer blank; 6) NK603 B73 BC0F1 7) LH82 x NK603 B73 BC1F1; 8) NK603 B73 BC1F1; 9) NK603 B73 BC5F1; 10) LH82 x NK603 B73 BC2F3; 11) B73 nontransgenic inbred control; 12) LH82 x B73 nontransgenic hybrid control.