Fig. 4. Western blot estimation of the amount of CP4 EPSPS L214P protein present in the isolated total CP4 EPSPS protein from NK603 grain and leaf tissue. Western blot probed with CP4 EPSPS L214P-specific antibodies. Lanes: 1) molecular weight marker; 2) 4 ng E. coli-expressed CP4 EPSPS; 3) 4 ng E. coli-expressed CP4 EPSPS L214P; 4) 2 ng E. coli-expressed CP4 EPSPS L214P; 5) 1 ng E. coli-expressed CP4 EPSPS L214P; 6) 0.5 ng E. coli-expressed CP4 EPSPS L214P; 7) 4 ng of total CP4 EPSPS purified from grain; 8) 4 ng of total CP4 EPSPS purified from leaves.