Fig. 4. PCR products from F₂ plants from the cross Newana x Redwin amplified by IRDye800-labeled primers 361SF and VA1-R and run on a Licor DNA Analyzer. Products were digested with restriction enzyme AciI to confirm that they originated in the A genome. Note that the specific primers for the ‘s’ allele amplified a product only from the 20 spring plants (1–20). The band is absent in the 10 winter individuals (21–30). Digestion with AciI produced the expected band of 116 bp, confirming the presence of a different ‘s’-specific polymorphism in the PCR products. M indicates the molecular weight marker.