Fig. 1. PCR amplification of primers VA1-F and VA1-R. DNAs were digested with restriction enzyme HinfI. Normal Chinese Spring in the first lane is followed by nulli-tetrasomic lines missing chromosomes 5A, 5B, and 5D, respectively. Note that there is no amplification product in the nulli-5A line, confirming that the primers are A-genome specific. The band size after restriction with HinfI was 690 bp as predicted by the A genome sequence.