Fig. 2. Zymograms of ß-amylase in cultivated barley. Isoelectric focusing was done with a Phastsystem (Pharmcia, Sweden) and ready-made acrylamide gel (PhastGEL IEF 4.0-6.5; Amersham Bioscience, Uppsala, Sweden). Lane 1: Harrington; 2: OUNI096; 3: OUC1152; 4: Haruna Nijo; 5: OUI429; 6: OUI462; 7: OUU421.