Fig. 5. Segregation of amplification products of microsatellite locus Xgdm132 in a silver-stained polyacrylamide gel (12%) after polymerase chain reaction. DNA of 97 DH lines from a cross between the resistant wheat cultivar Israel 493 (P₁) and the susceptible line RAC875-2 (P₂) was evaluated; R = resistant, and S = susceptible to an Australian isolate of the Septoria tritici blotch pathogen, M. graminicola. A 25-bp DNA ladder was used as a standard size marker. The 150-bp DNA fragment amplified from the resistant parent Israel 493 and resistant lines is indicated by the arrow on the right. Two larger bands also amplified in each progeny line but gave the same pattern as the 150-bp band, so were considered to represent the same allele.