Fig. 4. Friabilin proteins from ‘Chinese Spring’ (CS) and the lines with multiple copies of Pina and Pinb genes. The friabilin levels indicated by the upper Pina and lower Pinb bands show an increased concentration of friabilin in the lines 12, 30, and 284, each with three copies of the puroindoline genes. Lines 4 and 127 with two copies produce less protein, but are slightly more concentrated than the 1x (two copy) CS standard. Line 196 with two copies shows increased friabilin levels above that of other two-copy lines. The numbers in parentheses indicate puroindoline copy number.