Fig. 3. Starch surface protein levels, though not normalized, show a clear increase in starch surface protein levels that can be seen in lines 6, 12, 4, and 127, as indicated by the 15Mr (relative mobility) fragment. Starch surface protein is absent in the durum cultivar Langdon (L), but reappears at similar levels to ‘Chinese Spring’ (CS) in the Langdon 5D(5B) substitution line L5D(5B) that contains the 5D chromosome of CS. The numbers in parentheses indicate puroindoline copy number.