Fig. 2. Puroindoline expression analysis. RNA was extracted from 20-d post anthesis seeds. Membranes were probed with Pina and Pinb. Glutenin levels were used to normalize loading differences. ‘Chinese Spring’ (CS) was loaded in concentrations from 0.5x (one copy) to 4x (eight copies) as a reference. The adjusted values indicate a graphical representation of the expression levels compared with CS standards. The numbers in parentheses indicate puroindoline copy number.