Fig. 4. Expression of selected cloned genes in mature lemma/palea and various leaves of barley. Each lane contained 10 µg total RNA. The RNA blot was hybridized with ³²P-labeled 1-5, 1-14, 1-25, 1-52, 1-56, 1-107, 2-6, and 2-24 probes. EtBr stained agarose-formaldehyde gel is included at the bottom of each figure to show equal loading. L/P, pooled lemma/palea RNA from the middle (gelatinous) stage of kernel development; FL, flag leaf; 6, 6th leaf; 5, 5th leaf; 4, 4th leaf.