Fig. 3. Northern analysis of randomly selected clones confirms high expression levels in the lemma/palea compared with the flag leaf. Lemma and palea from the elongation, gelatinous and dough kernel stages were included to evaluate temporal expression patterns. Each lane contained 10 µg total RNA. The RNA blot was hybridized with ³²P-labeled probes 1-5, 1-25, 1-52, 2-6, 2-24 and 3-13. EtBr stained agarose-formaldehyde gel is included at the bottom of each figure to show equal loading. 1, 2, and 3 represent pooled lemma/palea RNA from elongation, gelatinous and early dough stages of kernel development, respectively; FL, flag leaf.